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To describe the manual method for determining the PT-INR on citrated plasma (blue tube). 2. SCOPE. 2.1 All staff who perform the manual INR method.PROCEDURE. A. Manual Method. 1. Bring all reagents, controls and sample to room temperature 15 minutes prior to testing. 2. Pre-warm PT reagent at 37°C for MANUAL PROTIME. The Prothrombin time is a useful screening procedure for deficiencies in factors II, V, VII and X. Deficiencies in factor I, although rare, Specific Manual Physical Therapy Techniques · Soft Tissue Mobilization. It is important to recognize the role of muscles and their attachments around the joints.